Improved Susceptibility Disk Assay Method Employing an Agar Overlay Technique
نویسندگان
چکیده
منابع مشابه
Improved susceptibility disk assay method employing an agar overlay technique.
Susceptibility disks impregnated with 35 different antibiotics were assayed by the current Code of Federal Regulations method and by a new method employing an agar overlay. The new method, a simplified analytical procedure, exhibited improvement in zone definition and an increase in dose response. Assays performed by the new method were accurate and precise. The agar overlay technique appears t...
متن کاملAgar-based disk diffusion assay for susceptibility testing of dermatophytes.
Currently, no agar-based susceptibility testing method has been standardized for testing dermatophytes. We describe a newly developed agar-based method employing disk diffusion assay to test the susceptibility of 47 isolates of dermatophytes against 8 antifungals. Our results show that the method is reproducible, is simple, and could be used to determine the antifungal susceptibility of dermato...
متن کاملMezlocillin susceptibility tests by the single-disk agar diffusion method.
A regression line for correlating zones of inhibition of a 75-mug mezlocillin disk with minimal inhibitory concentrations of mezlocillin for Enterobacteriaceae and nonfermenters was calculated. Correlation was excellent (r = -0.96) for all species tested except Pseudomonas cepacia.
متن کاملImproved agar gradient-plate technique.
MIC of antimicrobial compounds, it is essential to have uniform microbial growth on the agar medium; otherwise, it is difficult to determine a sharply defined area of growth inhibition. The conventional agar gradient-plate method has been found to be unsatisfactory for determining the MIC of chemotherapeutic compounds or antibiotics which inhibit filamentous fungi because the latter frequently ...
متن کاملCorrelation of netilmicin disk diffusion susceptibility and agar dilution susceptibility.
The minimal inhibitory concentration of netilmicin determined by the agar dilution method was correlated with the disk diffusion zone of inhibition against 322 clinical isolates. Regression line analysis revealed good correlation, r = -0.90, and suggested that isolates with zone sizes </=14 mm should be considered resistant.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1978
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.14.5.761